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Literature summary for 2.7.1.4 extracted from
- Partial purification and characterization of fructokinase activity from barley leaves (1989), J. Plant Physiol., 134, 156-161.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | severe inhibition with fructokinase-Ia and-Ib, slight with fructokinase II; substrate inhibition | Hordeum vulgare |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
D-fructose | fructokinase Ib, pH 7.6, 25°C | Hordeum vulgare | |
| 0.012 | - |
D-fructose | fructokinase II, pH 7.6, 25°C | Hordeum vulgare | |
| 0.12 | - |
D-fructose | fructokinase Ia, pH 7.6, 25°C | Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Hordeum vulgare | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulates | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
fructokinase-Ia, and fructokinase-1b, gel filtration | Hordeum vulgare |
| 73000 | - |
fructokinase II, gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Hordeum vulgare | - |
ADP + D-fructose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
L. cv. Brant, barley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 3 forms: fructokinase-Ia,-Ib,-II | Hordeum vulgare |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.8 | - |
fructokinase Ia and Ib | Hordeum vulgare |
| 60.3 | - |
fructokinase II | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Hordeum vulgare | ADP + D-fructose 6-phosphate | - |
? | |
| UTP + D-fructose | - |
Hordeum vulgare | UDP + D-fructose 6-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Hordeum vulgare |
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