Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | - |
Streptomyces violaceoruber | |
MgADP- | competitive | Streptomyces violaceoruber | |
MgATP2- | - |
Streptomyces violaceoruber |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ATP | ATP in form of MgATP2- | Streptomyces violaceoruber | |
0.5 | - |
D-fructose | - |
Streptomyces violaceoruber |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Streptomyces violaceoruber | |
Mg2+ | activation by free Mg2+ | Streptomyces violaceoruber | |
additional information | no activation by Mn2+ | Streptomyces violaceoruber | |
additional information | no activation by Cd2+ | Streptomyces violaceoruber | |
additional information | no activation by Cu2+ | Streptomyces violaceoruber |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Streptomyces violaceoruber | inducible enzyme | ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces violaceoruber | - |
- |
- |
Purification (Comment) | Organism |
---|---|
400fold | Streptomyces violaceoruber |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces violaceoruber | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces violaceoruber |
40 | - |
- |
Streptomyces violaceoruber |
Storage Stability | Organism |
---|---|
-20°C, in presence of Mg2+ and dithiothreitol, 25% loss of activity after 6 months | Streptomyces violaceoruber |
2°C, pH 7, in presence of Mg2+ and dithioerythritol, 25% loss of activity after 2 weeks | Streptomyces violaceoruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | specific for fructose | Streptomyces violaceoruber | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | inducible enzyme | Streptomyces violaceoruber | ADP + D-fructose 6-phosphate | - |
? | |
CTP + D-fructose | 30% of the activity with ATP | Streptomyces violaceoruber | CDP + D-fructose 6-phosphate | - |
? | |
dATP + D-fructose | 80% of the activity with ATP | Streptomyces violaceoruber | dADP + D-fructose 6-phosphate | - |
? | |
GTP + D-fructose | 50% of the activity with ATP | Streptomyces violaceoruber | GDP + D-fructose 6-phosphate | - |
? | |
ITP + D-fructose | 60% of the activity with ATP | Streptomyces violaceoruber | IDP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | 20% of the activity with ATP | Streptomyces violaceoruber | UDP + D-fructose 6-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 10 | lengthy plateau in the alkaline zone | Streptomyces violaceoruber |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
1 h, 50% loss of activity | Streptomyces violaceoruber |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
MgATP2- | - |
Streptomyces violaceoruber |