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Literature summary for 2.7.1.4 extracted from

  • Sabater, B.; Delafuente, G.
    Kinetic properties and related changes of molecular weight in a fructokinase from Streptomyces violaceoruber (1975), Biochim. Biophys. Acta, 377, 258-270.
    View publication on PubMed

General Stability

General Stability Organism
increased heat lability in presence of fructose Streptomyces violaceoruber

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibition above 1.5 mM MgCl2 at 1 mM ATP Streptomyces violaceoruber

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Streptomyces violaceoruber
Mg2+ required Streptomyces violaceoruber
additional information no activation by Ca2+ Streptomyces violaceoruber
additional information no activation by Cd2+ Streptomyces violaceoruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
glycerol gradient equilibrium sedimentation in presence of 10 mM fructose or 10 mM MgCl2 Streptomyces violaceoruber
80000
-
glycerol gradient equilibrium sedimentation in presence of 10 mM MgATP2- Streptomyces violaceoruber

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Streptomyces violaceoruber
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces violaceoruber
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Streptomyces violaceoruber

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
-
Streptomyces violaceoruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Streptomyces violaceoruber ADP + D-fructose 6-phosphate
-
?