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Literature summary for 2.7.1.39 extracted from

  • De Pascale, G.; Griffiths, E.; Shakya, T.; Nazi, I.; Wright, G.
    Identification and characterization of new inhibitors of fungal homoserine kinase (2011), ChemBioChem, 12, 1179-1182.
    View publication on PubMed
Search for more literature for 2.7.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
(2Z)-2-cyano-N-(2,5-dibromophenyl)-3-hydroxybut-2-enamide
-
 Schizosaccharomyces pombe
1-(naphthalen-2-yl)prop-2-en-1-one
-
 Schizosaccharomyces pombe
2-(2-methylpropyl)-6,7,8,9-tetrahydro-4H,5H-cyclohepta[4,5]thieno[2,3-d][1,3]oxazin-4-one
-
 Schizosaccharomyces pombe
2-(3,4-dihydroxyphenyl)-3,6,7-trihydroxy-2,3-dihydro-4H-chromen-4-one
-
 Schizosaccharomyces pombe
3-methyl-1-phenyl-6-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-4-ol
-
 Schizosaccharomyces pombe
4-hydroxy-3-[(4-hydroxy-2-oxo-3,8a-dihydro-2H-chromen-3-yl)methyl]-2H-chromen-2-one
-
 Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0676
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.463
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homoserine
-
 Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
Thr1
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.2
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
14.2
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00046
-
 4-hydroxy-3-[(4-hydroxy-2-oxo-3,8a-dihydro-2H-chromen-3-yl)methyl]-2H-chromen-2-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.0086
-
 3-methyl-1-phenyl-6-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-4-ol in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.012
-
 (2Z)-2-cyano-N-(2,5-dibromophenyl)-3-hydroxybut-2-enamide in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.0271
-
 2-(3,4-dihydroxyphenyl)-3,6,7-trihydroxy-2,3-dihydro-4H-chromen-4-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.032
-
 1-(naphthalen-2-yl)prop-2-en-1-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
0.0358
-
 2-(2-methylpropyl)-6,7,8,9-tetrahydro-4H,5H-cyclohepta[4,5]thieno[2,3-d][1,3]oxazin-4-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24.1
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
210
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe