Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.39 extracted from

  • Bareich, D.; Koteva, K.; Nazi, I.; Wright, G.D.
    Small molecule functional discrimination of the kinases required for the microbial synthesis of threonine and isoleucine (2004), Bioorg. Med. Chem., 12, 807-815.
    View publication on PubMed
Search for more literature for 2.7.1.39

Application

Application Comment Organism
medicine potential target for antibiotics due to the fact, that the pathway is not found in mammals Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 ATP pH 7.5, 30°C Schizosaccharomyces pombe
0.52
-
 L-homoserine pH 7.5, 30°C Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-homoserine Schizosaccharomyces pombe involved in the biosynthesis of threonine and isoleucine ADP + O-phospho-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Schizosaccharomyces pombe

Storage Stability

Storage Stability Organism
-80°C, 20 mM HEPES, pH 8.0, 10% glycerol, storage of purified enzyme Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homoserine
-
 Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?
ATP + L-homoserine involved in the biosynthesis of threonine and isoleucine Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40.2
-
 L-homoserine pH 7.5, 30°C Schizosaccharomyces pombe
40.8
-
 ATP pH 7.5, 30°C Schizosaccharomyces pombe