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Literature summary for 2.7.1.36 extracted from

  • Primak, Y.A.; Du, M.; Miller, M.C.; Wells, D.H.; Nielsen, A.T.; Weyler, W.; Beck, Z.Q.
    Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei (2011), Appl. Environ. Microbiol., 77, 7772-7778.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (lambdaDE3) cells Methanosarcina mazei

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited by dimethylallyl diphosphate, geranyl diphosphate, farnesyl diphosphate, isopentenyl monophosphate, and diphosphomevalonate Methanosarcina mazei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
(R)-mevalonate apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei
0.464
-
ATP apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
2 * 35500, calculated from amino acid sequence Methanosarcina mazei
78000
-
gel filtration Methanosarcina mazei

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap IMAC column chromatography and HiTrap Q column chromatography Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
Methanosarcina mazei ADP + (R)-5-phosphomevalonate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 35500, calculated from amino acid sequence Methanosarcina mazei

Synonyms

Synonyms Comment Organism
MVK
-
Methanosarcina mazei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3
-
(R)-mevalonate in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei