Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as dual-tagged His6-GST fusion protein | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphomevalonate | strong feedback inhibitor of the enzyme | Streptococcus pneumoniae | |
additional information | synthesis of a series of C3-substituted mevalonate analogues to probe the steric and electronic requirements of the mevalonate kinase active site for development of specific mechanism-based inhibitors, overview | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Streptococcus pneumoniae | - |
ADP + (R)-5-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q8DR51 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant dual-tagged His6-GST enzyme from Escherichia coli by affinity chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Streptococcus pneumoniae | ADP + (R)-5-phosphomevalonate | - |
? | |
additional information | the recombinant enzyme phosphorylates racemic vinyl, ethyl, n-propyl, n-butyl, i-butyl, 2-propenyl, allyl, ethynyl, and 1-propynyl mevalonate lactone analogues, that are diphosphorylated by phosphomevalonate kinase, EC 2.7.4.2, in a second step, overview | Streptococcus pneumoniae | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is regulating the mevalonate pathway in Streptococcus pneumoniae, it is strongly feedback-inhibited by diphosphomevalonate, the penultimate compound in the pathway, overview | Streptococcus pneumoniae |