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Literature summary for 2.7.1.36 extracted from
- Identification of catalytic residues in human mevalonate kinase (1997), J. Biol. Chem., 272, 25449-25454.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D204A | stable, with 40000fold diminution in kcat. Mutant is able to bind a spin-labeled ATP analogue with stoichiometries and equilibrium binding constants comparable to wild-type | Homo sapiens |
| D204N | stable, with 40000fold diminution in kcat. Mutant is able to bind a spin-labeled ATP analogue with stoichiometries and equilibrium binding constants comparable to wild-type | Homo sapiens |
| E193A | labile | Homo sapiens |
| E193Q | 50fold diminution in Vmax and 20fold increase Km values for ATP, 40fold increase in Km for mevalonate | Homo sapiens |
| E19A | destabilization | Homo sapiens |
| E19D | stable, decrease in Vmax to 40% of wild-type | Homo sapiens |
| E19Q | destabilization | Homo sapiens |
| E296Q | stable. Kinetic parameters similar to wild-type | Homo sapiens |
| H20A | insoluble upon expression in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
mevalonate | mutant D204N, pH 7.0, 30°C | Homo sapiens |  |
| 0.024 | - |
mevalonate | wild-type, pH 7.0, 30°C | Homo sapiens | |
| 0.025 | - |
ATP | mutant D204A, pH 7.0, 30°C | Homo sapiens | |
| 0.052 | - |
mevalonate | mutant E19D, pH 7.0, 30°C | Homo sapiens | |
| 0.066 | - |
ATP | mutant D204N, pH 7.0, 30°C | Homo sapiens | |
| 0.074 | - |
ATP | wild-type, pH 7.0, 30°C | Homo sapiens | |
| 0.11 | - |
mevalonate | mutant E296Q, pH 7.0, 30°C | Homo sapiens | |
| 0.113 | - |
ATP | mutant E19D, pH 7.0, 30°C | Homo sapiens | |
| 0.131 | - |
ATP | mutant E296Q, pH 7.0, 30°C | Homo sapiens | |
| 0.2 | - |
mevalonate | mutant D204A, pH 7.0, 30°C | Homo sapiens | |
| 1.02 | - |
mevalonate | mutant E193Q, pH 7.0, 30°C | Homo sapiens | |
| 1.56 | - |
ATP | mutant E193Q, pH 7.0, 30°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Homo sapiens |
| 78000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate | role for residue E193 in interacting with the cation of the MgATP substrate, and for D204 as the catalytic base that facilitates deprotonation of the C5 hydroxyl of mevalonic acid | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 7.0, 30°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | - |
Homo sapiens | ADP + phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Homo sapiens |
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Release 2023.1 (January 2023)
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