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Literature summary for 2.7.1.36 extracted from

  • Chu, X.; Yu, W.; Wu, L.; Liu, X.; Li, N.; Li, D.
    Effect of a disulfide bond on mevalonate kinase (2007), Biochim. Biophys. Acta, 1774, 1571-1581.
    View publication on PubMed
Search for more literature for 2.7.1.36

Protein Variants

Protein Variants Comment Organism
A141C significantly higher thermal activity than wild-type Rattus norvegicus
C107A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
C107S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
C107S/C281S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
C197A/C281A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
C281A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
C281S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Rattus norvegicus
0.061
-
 (R)-mevalonate mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
0.07
-
 (R)-mevalonate mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
0.071
-
 (R)-mevalonate mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
0.076
-
 (R)-mevalonate mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
0.106
-
 (R)-mevalonate mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
0.106
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii
0.111
-
 (R)-mevalonate mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
0.186
-
 (R)-mevalonate mutant A141C, pH 7.5, 34°C Rattus norvegicus
0.95
-
 ATP wild-type, pH 7.5, 34°C Rattus norvegicus
0.97
-
 ATP mutant A141C, pH 7.5, 34°C Rattus norvegicus
1.18
-
 ATP wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii
1.58
-
 ATP mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.61
-
 ATP mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.76
-
 ATP mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.97
-
 ATP mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
3.11
-
 ATP mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
3.36
-
 ATP mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
 Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
 ?
ATP + (R)-mevalonate
-
 Methanocaldococcus jannaschii ADP + (R)-5-phosphomevalonate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
72
-
 wild-type Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
34
-
 wild-type, 25% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 10 min, residual activity of wild-type 50%, of mutant A141C 85% Rattus norvegicus
47
-
 10 min, residual activity of wild-type 15%, of mutant A141C 55% Rattus norvegicus
83
-
 10 min, residual activity of wild-type 50%, mutants C107S and C281S 20%, mutant C107S/C281S 15%, mutants C107A and C281A 20%, mutant C107A/C281A 18% Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 (R)-mevalonate mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
5.2
-
 (R)-mevalonate mutant A141C, pH 7.5, 34°C Rattus norvegicus
8.6
-
 (R)-mevalonate mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
10.5
-
 (R)-mevalonate mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
12.7
-
 (R)-mevalonate mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
17.1
-
 (R)-mevalonate mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
21.9
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Rattus norvegicus
23.3
-
 (R)-mevalonate mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
28.5
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii