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Literature summary for 2.7.1.36 extracted from

  • Hedl, M.; Rodwell, V.W.
    Enterococcus faecalis mevalonate kinase (2004), Protein Sci., 13, 687-693.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine potential target for drugs Enterococcus faecalis

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Enterococcus faecalis
additional information no inhibition with AMP Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
GTP pH 10, 37°C Enterococcus faecalis
0.33
-
(R)-mevalonate pH 10, 37°C Enterococcus faecalis
0.8
-
CTP pH 10, 37°C Enterococcus faecalis
1.1
-
ATP pH 10, 37°C Enterococcus faecalis
1.4
-
ITP pH 10, 37°C Enterococcus faecalis
1.9
-
TTP pH 10, 37°C Enterococcus faecalis
2.8
-
UTP pH 10, 37°C Enterococcus faecalis
3.3
-
Mg2+ pH 10, 37°C Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Enterococcus faecalis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace for Mg2+ Enterococcus faecalis
Mg2+ required Enterococcus faecalis
Mn2+ can replace for Mg2+ Enterococcus faecalis
Zn2+ can replace for Mg2+ Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32200
-
sedimentation velocity Enterococcus faecalis
33840
-
calculated from the deduced amino acid sequence Enterococcus faecalis
71100
-
sedimentation velocity, 10% of total enzyme Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Enterococcus faecalis involved in isopentenyl diphosphate synthesis ADP + (R)-5-phosphomevalonate
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Enterococcus faecalis

Reaction

Reaction Comment Organism Reaction ID
ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate sequential mechanism Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
purified enzyme, pH 10, 37°C Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
Enterococcus faecalis ADP + (R)-5-phosphomevalonate
-
?
ATP + (R)-mevalonate involved in isopentenyl diphosphate synthesis Enterococcus faecalis ADP + (R)-5-phosphomevalonate
-
?
CTP + (R)-mevalonate
-
Enterococcus faecalis CDP + (R)-5-phosphomevalonate
-
?
GTP + (R)-mevalonate
-
Enterococcus faecalis GDP + (R)-5-phosphomevalonate
-
?
ITP + (R)-mevalonate
-
Enterococcus faecalis IDP + (R)-5-phosphomevalonate
-
?
TTP + (R)-mevalonate
-
Enterococcus faecalis TDP + (R)-5-phosphomevalonate
-
?
UTP + (R)-mevalonate
-
Enterococcus faecalis UDP + (R)-5-phosphomevalonate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32200, sedimentation velocity, 10% of total enzyme Enterococcus faecalis
monomer 1 * 32200, sedimentation velocity, SDS-PAGE Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Enterococcus faecalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45
-
Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Enterococcus faecalis

pH Range

pH Minimum pH Maximum Comment Organism
8 11
-
Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7
-
ADP pH 10, 37°C Enterococcus faecalis