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Literature summary for 2.7.1.36 extracted from
- Purification and characterization of mevalonate kinase from suspension-cultured cells of Catharanthus roseus (L.) G. Don (2000), Arch. Biochem. Biophys., 378, 287-298.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | product inhibition vs. ATP at concentration above 2 mM | Catharanthus roseus |  |
| farnesyl diphosphate | competitive vs. ATP | Catharanthus roseus | |
| mevalonate 5-phosphate | noncompetitive vs. ATP, mixed-type vs. mevalonate | Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
mevalonate | pH 7.5, 30°C | Catharanthus roseus | |
| 0.13 | - |
ATP | pH 7.5, 30°C | Catharanthus roseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | approx. 18% of activity with Mg2+ or Mn2+ at 2 mM | Catharanthus roseus | |
| Fe2+ | approx. 30% of activity with Mg2+ or Mn2+ at 2 mM | Catharanthus roseus | |
| Mn2+ | divalent cation required for activity | Catharanthus roseus | |
| Mn2+ | as effectiv as Mg2+ | Catharanthus roseus | |
| Zn2+ | approx. 35% of activity with Mg2+ or Mn2+ at 2 mM | Catharanthus roseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41500 | - |
2 * 41500, SDS-PAGE | Catharanthus roseus |
| 101900 | - |
additional bands at 19100, 23500, 45100, 73500 and 271200 Da, native PAGE | Catharanthus roseus |
| 104600 | - |
gel filtration | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | Catharanthus roseus | via mevalonate pathway involved in isoprenoid biosynthesis | ADP + phosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
(L.) G. Don | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, Phenyl-Sepharose, Mono Q, Shodex KW 803 | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.6 | - |
- |
Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | - |
Catharanthus roseus | ADP + phosphomevalonate | - |
? | |
| ATP + mevalonate | via mevalonate pathway involved in isoprenoid biosynthesis | Catharanthus roseus | ADP + phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41500, SDS-PAGE | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
broad optimum between pH 7.0 and pH 10.0 | Catharanthus roseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | approx. 10% of maximal activity at pH 5.0, approx. 90% of maximal activity at pH 10.0 | Catharanthus roseus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
farnesyl diphosphate | pH 7.5, 30°C | Catharanthus roseus | |
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