Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
farnesyl diphosphate | 0.01 mM, 65% inhibition | Methanocaldococcus jannaschii | |
geranyl diphosphate | 0.01 mM, 55% inhibition | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
(R,S)-mevalonate | pH 8.0, 70°C, recombinant mevalonate kinase | Methanocaldococcus jannaschii | |
0.092 | - |
ATP | pH 8.0, 70°C, recombinant mevalonate kinase | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
68000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + mevalonate | Methanocaldococcus jannaschii | involved in mevalonate pathway of terpenoid biosynthesis | ADP + phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mevalonate kinase, 70°C heat treatment, Talon metal-affinity resin | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
387 | - |
recombinant mevalonate kinase | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + mevalonate | - |
Methanocaldococcus jannaschii | ADP + phosphomevalonate | - |
? | |
ATP + mevalonate | involved in mevalonate pathway of terpenoid biosynthesis | Methanocaldococcus jannaschii | ADP + phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | - |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 90 | approx. 25% of maximal activity at 40°C and 90°C, respectively | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no loss of activity after 24 h | Methanocaldococcus jannaschii |
90 | - |
50% loss of activity after 15 min | Methanocaldococcus jannaschii |
100 | - |
50% loss of activity after 5 min | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | at least 60% of maximal activity between pH 5.0 and 7.0 | Methanocaldococcus jannaschii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanocaldococcus jannaschii | MonoP chromatography | - |
7.8 |
Methanocaldococcus jannaschii | calculated from amino acid sequence | - |
8.4 |