Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 10-15% (w/v) PEG 8000, 0.05-0.1 M NaOAc and 0.05 M NaCl dissolved in 0.1 M sodium cacodylate buffer pH 6.5 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | feedback inhibitor | Escherichia coli | |
CoA | feedback inhibitor | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6I3 | - |
- |
Mycobacterium tuberculosis | P9WPA7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPA7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA metal affinity column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Mycobacterium tuberculosis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Escherichia coli | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Mycobacterium tuberculosis H37Rv | ADP + (R)-4'-phosphopantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PanK | - |
Mycobacterium tuberculosis |
PanK | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis | |
ATP | - |
Escherichia coli |