Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of the His-tagged in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.003 ml protein solution containing 6 mg/ml protein, 0.1 M Tris-HCl, pH 8.0, 0.15 M NaCl, 5% v/v glycerol and 0.001 M 2-mercaptoethanol, mixed 0.001 ml of precipitant solution containing 10-15% w/v PEG 8000, 0.05-0.1 M NaOAc, 0.05 NaCl, and 0.1 M cacodylate, pH 6.5, room temperature, equilibration against 0.4 ml precipitant solution, 3-7 days, 2 different crystal forms, X-ray diffraction structure determination and analysis at 2.5 A and 2.9 A resolution, respectively, molecular replacement calculations | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
x * 37500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Mycobacterium tuberculosis | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | Mycobacterium tuberculosis H37Rv | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Mycobacterium tuberculosis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | i.e. vitamin B5 | Mycobacterium tuberculosis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Mycobacterium tuberculosis H37Rv | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | i.e. vitamin B5 | Mycobacterium tuberculosis H37Rv | ADP + (R)-4'-phosphopantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PanK | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |