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Literature summary for 2.7.1.33 extracted from

  • Vallari, D.S.; Jackowski, S.; Rock, C.O.
    Regulation of pantothenate kinase by coenzyme A and its thioesters (1987), J. Biol. Chem., 262, 2468-2471.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
Escherichia coli
acetyl-CoA
-
Escherichia coli
ADP
-
Escherichia coli
AMP
-
Escherichia coli
CoA in vitro; in vivo; kinetics Escherichia coli
dephospho-CoA not Escherichia coli
malonyl-CoA
-
Escherichia coli
additional information not inhibitory: 3',5'-ADP; not inhibitory: cAMP, NADH, NADPH, CoA:glutathione disulfide; not inhibitory: NAD+ Escherichia coli
palmitoyl-CoA
-
Escherichia coli
pantetheine 4'-phosphate not Escherichia coli
succinyl-CoA
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
pantothenate
-
Escherichia coli
0.225
-
ATP pH 7.4, 25°C Escherichia coli
0.225
-
ATP ATP in form of MgATP2- Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Escherichia coli 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-pantothenate Escherichia coli first and rate-controlling reaction of CoA-biosynthesis ADP + D-4'-phosphopantothenate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12 strains UB 1005, SJ16, DV1
-

Purification (Commentary)

Purification (Comment) Organism
strain SJ16, partial Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, partially purified preparation, several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
Escherichia coli ADP + (R)-4'-phosphopantothenate
-
?
ATP + D-pantothenate poor phosphate donor: alpha,beta-methyleneadenosine 5'-triphosphate Escherichia coli ADP + D-4'-phosphopantothenate
-
?
ATP + D-pantothenate transfers the gamma-phosphate of ATP to pantothenate Escherichia coli ADP + D-4'-phosphopantothenate
-
?
ATP + D-pantothenate first and rate-controlling reaction of CoA-biosynthesis Escherichia coli ADP + D-4'-phosphopantothenate
-
?
GTP + pantothenate phosphorylation at 20% the rate of ATP Escherichia coli GDP + D-4'-phosphopantothenate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli