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Literature summary for 2.7.1.33 extracted from

  • Abiko, Y.; Ashida, S.I.; Shimizu, M.
    Purification and properties of D-pantothenate kinase from rat liver (1972), Biochim. Biophys. Acta, 268, 364-372.
    View publication on PubMed
Search for more literature for 2.7.1.33

General Stability

General Stability Organism
ATP and/or sucrose stabilize during purification Rattus norvegicus
considerably unstable independent of the degree of purity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2'-Ketopantetheine
-
 Rattus norvegicus
CoA strong Rattus norvegicus
dephospho-CoA
-
 Rattus norvegicus
L-Pantothenate
-
 Rattus norvegicus
pantetheine 4'-phosphate strong Rattus norvegicus
pantothenoylcysteine 4'-phosphate strong Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 pantothenate pH 6.1, 37°C Rattus norvegicus
1
-
 ATP pH 6.1, 37°C Rattus norvegicus
1
-
 ATP ATP in form of MgATP2- Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, active substrate: MgATP-complex Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-pantothenate Rattus norvegicus first and rate-controlling reaction of CoA-biosynthesis ADP + D-4'-phosphopantothenate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Wistar
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0041
-
 pH 6.1, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, above 3 mg protein/ml, more than 1 week Rattus norvegicus
4°C, above 3 mg protein/ml, 4 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Rattus norvegicus ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + D-pantothenate D-configuration of 2'-hydroxyl group in pantothenate molecule is essential for functional interaction with enzyme Rattus norvegicus ADP + D-4'-phosphopantothenate
-
 ?
ATP + D-pantothenate first and rate-controlling reaction of CoA-biosynthesis Rattus norvegicus ADP + D-4'-phosphopantothenate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9 6.3
-
 Rattus norvegicus