Application | Comment | Organism |
---|---|---|
medicine | the enzyme is implicated in tumorigenesis and an attractive target for anticancer chemotherapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
isoform ChoKalpha1 in complex with hemicholinium-3 and ADP, sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 25% (w/v) polyethylene glycol 3350, and 0.2 M lithium sulfate | Homo sapiens |
isoform ChoKbeta in complex with hemicholinium-3 and ADP, sitting drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 30% polyethylene glycol 4000, and 0.2 M ammonium sulfate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F352A | the mutant exhibits a higher turnover number compared to the wild type enzyme | Homo sapiens |
F352L | the mutant exhibits a higher turnover number compared to the wild type enzyme | Homo sapiens |
L401A | the mutant of isoform ChoKalpha1 exhibts a lower turnover number compared to the wild type enzyme | Homo sapiens |
L401F | the mutant of isoform ChoKalpha1 exhibts a lower turnover number compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hemicholinium-3 | competitive inhibitor, the inhibitory effect of hemicholinium-3 is about 500times higher toward the ChoKalpha isoforms than ChoKbeta isoform; competitive inhibitor, the inhibitory effect of hemicholinium-3 is about 500times higher toward the ChoKalpha isoforms than ChoKbeta isoform | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
choline | isoform ChoKalpha2 mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.033 | - |
choline | wild type isoform ChoKbeta, pH and temperature not specified in the publication | Homo sapiens | |
0.041 | - |
choline | isoform ChoKbeta mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.086 | - |
choline | wild type isoform ChoKalpha2, pH and temperature not specified in the publication | Homo sapiens | |
0.102 | - |
choline | isoform ChoKbeta mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.107 | - |
choline | isoform ChoKalpha2 mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.18 | - |
choline | wild type isoform ChoKalpha1, pH and temperature not specified in the publication | Homo sapiens | |
0.4 | - |
ATP | isoform ChoKalpha2 mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.4 | - |
ATP | wild type isoform ChoKalpha2, pH and temperature not specified in the publication | Homo sapiens | |
0.4 | - |
ATP | wild type isoform ChoKbeta, pH and temperature not specified in the publication | Homo sapiens | |
0.55 | - |
ATP | isoform ChoKalpha2 mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.65 | - |
ATP | isoform ChoKbeta mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.7 | - |
ATP | isoform ChoKbeta mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.76 | - |
ATP | wild type isoform ChoKalpha1, pH and temperature not specified in the publication | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35790 | subunit CKalpha | - |
Homo sapiens | Q9Y259 | subunit CKbetha | - |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Homo sapiens | ADP + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHOKalpha1 | isoform | Homo sapiens |
ChoKalpha2 | isoform | Homo sapiens |
ChoKbeta1 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.92 | - |
choline | wild type isoform ChoKbeta, pH and temperature not specified in the publication | Homo sapiens | |
4.58 | - |
choline | isoform ChoKbeta mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
9.5 | - |
choline | isoform ChoKbeta mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
14 | - |
choline | isoform ChoKalpha2 mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
47.33 | - |
choline | wild type isoform ChoKalpha2, pH and temperature not specified in the publication | Homo sapiens | |
49.5 | - |
choline | isoform ChoKalpha2 mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
76.83 | - |
choline | wild type isoform ChoKalpha1, pH and temperature not specified in the publication | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
hemicholinium-3 | wild type isoform ChoKalpha1, pH and temperature not specified in the publication | Homo sapiens | |
0.00023 | - |
hemicholinium-3 | wild type isoform ChoKalpha2, pH and temperature not specified in the publication | Homo sapiens | |
0.00045 | - |
hemicholinium-3 | isoform ChoKalpha2 mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.005 | - |
hemicholinium-3 | isoform ChoKbeta mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.008 | - |
hemicholinium-3 | isoform ChoKbeta mutant F352A, pH and temperature not specified in the publication | Homo sapiens | |
0.016 | - |
hemicholinium-3 | isoform ChoKalpha2 mutant F352L, pH and temperature not specified in the publication | Homo sapiens | |
0.116 | - |
hemicholinium-3 | wild type isoform ChoKbeta, pH and temperature not specified in the publication | Homo sapiens |