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Literature summary for 2.7.1.32 extracted from
- Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion (2008), J. Biol. Chem., 283, 10079-10088.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | intacellular zinc depletion resulted in a concentration-dependent induction of CKI1 expression | Saccharomyces cerevisiae |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid maintenance and amplification are performed in Escherichia coli strain DH5 alpha | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + ATP | Saccharomyces cerevisiae | The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo and in an increase in phosphatidylcholine synthesis via the Kennedy pathway | phosphocholine + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| yeast form | strains W303-1A,DY1457, ZHY6, ZHY3, SH304, SH303, SH307 | Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
The specific activity of choline kinase in wild type zinc-depleted cells is 2.7fold greater when compared with the activity from cells grown with 1.5 microM ZnSO4. Choline kinase activity is measured in a reaction mixture that contains 67 mM glycine-NaOH, 5 mM (methyl-14C)choline, 5 mM ATP, 10mM MgSO4 and 1.3 mM dithiothreitol. The radiolabeled product phosphocholine, is separated from the substrate by precipitation of choline as a reineckate salt. | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + ATP | The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo and in an increase in phosphatidylcholine synthesis via the Kennedy pathway | Saccharomyces cerevisiae | phosphocholine + ADP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| choline kinase | - |
Saccharomyces cerevisiae |
| CKI1 | - |
Saccharomyces cerevisiae |
| CKI1-encoded choline kinase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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