Literature summary for 2.7.1.32 extracted from

Glunde, K.; Shah, T.; Winnard, P.T.; Raman, V.; Takagi, T.; Vesuna, F.; Artemov, D.; Bhujwalla, Z.M.
Hypoxia regulates choline kinase expression through hypoxia-inducible factor-1 alpha signaling in a human prostate cancer model (2008), Cancer Res., 68, 172-180.

Application

Application	Comment	Organism
drug development	choline kinase is gaining importance as a potential target for anticancer therapy	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
The genomic sequence of the putative human chk-alpha promoter is obtained by performing a BLAST search in National Center for Biotechnology Information using the known promoter sequence of the rat chk-a gene as query sequence. A 2.3-kb region at the 5'-end of the human chk-a gene on chromosome 11, immediately upstream of the translation start site, is found to contain several putative HRE sites with the core sequence RCGTG. Due to its high GC content, this 2.3-kb region is cloned as four smaller overlapping fragments, and formamide (5%) is added in all PCRs. The four overlapping sequences are PCR amplified from human genomic DNA. The PCR products of paired sense and antisense primer sequences used for the cloning of these fragments (Chk) are subcloned into the pCR 2.1 TA vector. The full 2.3-kb promoter region is obtained by appropriate endonuclease digestions and ligation of these four overlapping fragments. Each of the four fragments alone, as well as several subligations of two or three consecutive fragments, and the full-length 2.3-kb chk-a promoter are cloned into the pGL4-basic Luc reporter vector.	Homo sapiens

Cloned (Comment)

Organism

The genomic sequence of the putative human chk-alpha promoter is obtained by performing a BLAST search in National Center for Biotechnology Information using the known promoter sequence of the rat chk-a gene as query sequence. A 2.3-kb region at the 5'-end of the human chk-a gene on chromosome 11, immediately upstream of the translation start site, is found to contain several putative HRE sites with the core sequence RCGTG. Due to its high GC content, this 2.3-kb region is cloned as four smaller overlapping fragments, and formamide (5%) is added in all PCRs. The four overlapping sequences are PCR amplified from human genomic DNA. The PCR products of paired sense and antisense primer sequences used for the cloning of these fragments (Chk) are subcloned into the pCR 2.1 TA vector. The full 2.3-kb promoter region is obtained by appropriate endonuclease digestions and ligation of these four overlapping fragments. Each of the four fragments alone, as well as several subligations of two or three consecutive fragments, and the full-length 2.3-kb chk-a promoter are cloned into the pGL4-basic Luc reporter vector.

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	commentary	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
PC-3 cell	prostate cancer cell	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
CHK	-	Homo sapiens
chk-alpha	-	Homo sapiens
choline kinase	-	Homo sapiens