Application | Comment | Organism |
---|---|---|
drug development | choline kinase is elevated in cancer cells and presents a novel target for increasing cell kill. Data support exploring transient Chk silencing as a broad-spectrum chemotherapeutic agent for cancer cells. | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Small interfering RNA-chk (siRNA) is designed and transfected for 48 h using oligofectamine and Opti-MEM based on the observed decrease of Chk message using reverse transcription-PCR analysis within this time. Immunoblot assay results with Chk antibody shows significant reduction of Chk protein expression levels in MCF-12A, MCF-7, and MDA-MB-231 cells after transient siRNA-chk transfection. | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | Investigation of the effect of combining transient siRNA-chk transfection with the anticancer drug 5-fluorouracil on cell viability and proliferation. Choline kinase down-regulation with 5-fluorouracil treatment increased cell kill in cancer cells. | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-12A cell | HMEC cell line, a spontaneously immortalized nonmalignant mammary epithelial cell line | Homo sapiens | - |
MCF-7 cell | estrogen receptor-positive poorly metastatic breast cancer cell line | Homo sapiens | - |
MDA-MB-231 cell | invasive estrogen receptor-negative cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHK | - |
Homo sapiens |
choline kinase | - |
Homo sapiens |