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Literature summary for 2.7.1.32 extracted from

  • Mori, N.; Glunde, K.; Takagi, T.; Raman, V.; Bhujwalla, Z.M.
    Choline kinase down-regulation increases the effect of 5-fluorouracil in breast cancer cells (2007), Cancer Res., 67, 11284-11290.
    View publication on PubMed

Application

Application Comment Organism
drug development choline kinase is elevated in cancer cells and presents a novel target for increasing cell kill. Data support exploring transient Chk silencing as a broad-spectrum chemotherapeutic agent for cancer cells. Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
Small interfering RNA-chk (siRNA) is designed and transfected for 48 h using oligofectamine and Opti-MEM based on the observed decrease of Chk message using reverse transcription-PCR analysis within this time. Immunoblot assay results with Chk antibody shows significant reduction of Chk protein expression levels in MCF-12A, MCF-7, and MDA-MB-231 cells after transient siRNA-chk transfection. Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-fluorouracil Investigation of the effect of combining transient siRNA-chk transfection with the anticancer drug 5-fluorouracil on cell viability and proliferation. Choline kinase down-regulation with 5-fluorouracil treatment increased cell kill in cancer cells. Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Source Tissue

Source Tissue Comment Organism Textmining
MCF-12A cell HMEC cell line, a spontaneously immortalized nonmalignant mammary epithelial cell line Homo sapiens
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MCF-7 cell estrogen receptor-positive poorly metastatic breast cancer cell line Homo sapiens
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MDA-MB-231 cell invasive estrogen receptor-negative cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CHK
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Homo sapiens
choline kinase
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Homo sapiens