Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation of choline kinase is essential for building membranes, cell growth, cell proliferationand for rebuilding phospholipids that are degraded in the process of signal transductiony | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | Homo sapiens | - |
phosphocholine + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
MCF-7 cell | human breast cancer cell line | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
exponentially growing cells: 484.04 pmol/mg/min (activity is determined after homogenizing a cell pellet in Tris/HCl buffer. Homogenisate of exponentially growing and confluent cells is centrifugated. Assay mixture containing 40 mM Tris/HCl with unlabelled carrier of 0.25 mM choline, (methyl-3H)choline (37 kBq/sample), 2 mM ATP and 10 mM MgCl2 were added to homogenate protein. After incubation, reaction is stopped by addition of ice-cold mixture of methanol and chloroform and lipid-metabolite extraction is then carried out. Protein content is set for each sample and total radioactivity is determined. Separation of (methyl-3H)-phosphocholine and (methyl-3H)-choline is carried out using ion exchange chromatography) | Homo sapiens |
0.000484 | - |
in 40 mM Tris/HCl with 10 mM MgCl2, 2 mM ATP and 0.25 mM choline, pH 7.4, at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Homo sapiens | ADP + phosphocholine | - |
? | |
choline + ATP | - |
Homo sapiens | phosphocholine + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChoK | - |
Homo sapiens |
choline kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |