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Literature summary for 2.7.1.30 extracted from
- Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis (2000), Eur. J. Biochem., 267, 2323-2333.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A137S | affinity for substrates increased 3-4 fold | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
ATP | 25°C, pH 8.0 | Trypanosoma brucei |  |
| 0.44 | - |
glycerol | 25°C, pH 8.0 | Trypanosoma brucei | |
| 0.56 | - |
ADP | 25°C, pH 8.0 | Trypanosoma brucei | |
| 3.83 | - |
L-glycerol-3-phosphate | 25°C, pH 8.0 | Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, deduced from gene sequence | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei brucei | Q9NJP9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | - |
Trypanosoma brucei | ADP + sn-glycerol 3-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, deduced from gene sequence | Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Trypanosoma brucei |
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