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Literature summary for 2.7.1.25 extracted from

  • Satishchandran, C.; Markham, G.D.
    Mechanistic studies of Escherichia coli adenosine-5'-phosphosulfate kinase (2000), Arch. Biochem. Biophys., 378, 210-215.
    View publication on PubMed
Search for more literature for 2.7.1.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 adenosine 5'-phosphosulfate pH 8.0 Escherichia coli
0.06
-
 adenosine 5'-O-(3-thiotriphosphate) pH 8.0 Escherichia coli
0.13
-
 ADP ADP in form of MgADP- Escherichia coli
0.37
-
 3'-phosphoadenosine 5'-phosphosulfate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ affinity for adenosine 5'-phosphosulfate decreases by 15fold in the absence of Mg2+ Escherichia coli
Mn2+ 1 Mn2+ ion per subunit in the absence of substrate, 2 Mn2+ bind per subunit when APS or the ATP analog 5'-adenylylimidodiphosphate are present, afffinity for Mn2+ increases 23fold when the enzyme is phosphorylated Escherichia coli
additional information enzyme uses multiple metal ions to catalyze phosphoryl group transfer Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate kinetic scheme for APS kinase Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-O-(3-thiotriphosphate) + adenosine 5-phosphosulfate 535fold lower Vmax than with ATP Escherichia coli ADP + 3'-thiophosphoadenosine 5'-phosphosulfate
-
 ?
ADP + 3'-phosphoadenylyl sulfate
-
 Escherichia coli ATP + adenylyl sulfate
-
 r
ATP + adenosine 5'-O-(2-fluorodiphosphate) 15fold lower Vmax than with adenosine 5-phosphosulfate Escherichia coli ADP + 3'-phosphoadenosine 5'-O-(2-fluorodiphosphate)
-
 ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 adenosine 5'-O-(3-thiotriphosphate) pH 8.0 Escherichia coli