Literature summary for 2.7.1.25 extracted from
Schwenn, J.D.; Jender, H.G.
A kinetic investigation of the APS-kinase from chlamydomonas reinhardii CW 15 (1981), Phytochemistry, 20, 601-604.
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
adenosine 5'-phosphosulfate |
substrate inhibition above 0.03-0.04 mM |
Chlamydomonas reinhardtii |
|
ATP |
in the absence of ATP regenerating system, substrate inhibition above 0.6 mM, in the presence of ATP regenerating system, substrate inhibition above 0.2 mM |
Chlamydomonas reinhardtii |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.006 |
- |
adenosine 5'-phosphosulfate |
pH 8.0 |
Chlamydomonas reinhardtii |
|
0.05 |
0.06 |
ATP |
pH 8.0 |
Chlamydomonas reinhardtii |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
activation |
Chlamydomonas reinhardtii |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Chlamydomonas reinhardtii |
- |
CW15, cell wall mutant |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
ammonium sulfate, Sephacryl S-200, partially purified |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
0.002 |
0.006 |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + adenosine 5-phosphosulfate |
i.e. adenylylsulfate or APS |
Chlamydomonas reinhardtii |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate |
r |
|