Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activation | Rattus norvegicus |
General Stability | Organism |
---|---|
stable to ammonium sulfate precipitation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | potent substrate inhibition, ATP reverses | Rattus norvegicus | |
iodoacetamide | strong inhibition, dithiothreitol partially protects | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14500 | - |
4 * 14500, SDS-PAGE | Rattus norvegicus |
58000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
alumina C-gamma gel, ammonium sulfate, hydroxyapatite, Cellex CM, unstable upon column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52.5 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Rattus norvegicus | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 14500, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | approx. half-maximal activity at pH 7.5, approx. 80% of maximal activity at pH 9.0 | Rattus norvegicus |