Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D32A | the mutant shows a catalytic efficiency of only 5% of the native enzyme | Mycobacterium tuberculosis |
D32E | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
D32N | the mutant shows an almost 2.54times increased Km value for 3'-dephospho-CoA compared to the wild type enzyme. The absence of magnesium completely ablates activity for the D32N mutant | Mycobacterium tuberculosis |
G8A | this mutation does not change either the Km or the Kcat of the reaction considerably | Mycobacterium tuberculosis |
K14A | the substitution affects the kinetic parameters of the reaction resulting in a mere 19% reduction in the Kcat of the enzyme, the mutant demonstrates a 50% increase in the Km for ATP | Mycobacterium tuberculosis |
L114A | the mutation results in a decrease in the affinity of the enzyme for the acceptor substrate | Mycobacterium tuberculosis |
R140A | this mutation completely abolishes kinetic activity | Mycobacterium tuberculosis |
R140K | the mutation results in a dramatic loss of catalytic activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0217 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0345 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0349 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0372 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0501 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0568 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0575 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0598 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0639 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0758 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0809 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0867 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0877 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.181 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.501 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPA3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3'-dephospho-CoA | - |
Mycobacterium tuberculosis | ADP + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CoaE | - |
Mycobacterium tuberculosis |
dephosphocoenzyme A kinase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0076 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.014 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0192 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0255 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0257 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0293 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0327 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0334 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.039 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.043 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0481 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0569 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0651 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.067 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.0015 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.042 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.243 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.3 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.337 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.38 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.448 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.69 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.703 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.713 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.838 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
0.847 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
1.3 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
1.93 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis |