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Literature summary for 2.7.1.24 extracted from
- Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase) (2002), Biochem. J., 365, 13-18.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ppat/dpck encoding bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activity, located on chromosome 17q12-21, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0041 | - |
dephospho-CoA | native enzyme, pH 8.0, 25°C | Sus scrofa |  |
| 0.0052 | - |
dephospho-CoA | recombinant enzyme, pH 8.0, 25°C | Homo sapiens | |
| 0.19 | - |
ATP | recombinant enzyme, pH 8.0, 25°C | Homo sapiens | |
| 0.33 | - |
ATP | recombinant enzyme, pH 8.0, 25°C | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
1 * 60000, SDS-PAGE | Sus scrofa |
| 62000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
| 62000 | - |
wild-type enzyme, gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q13057 | gene ppat/dpck, bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities, termed CoA synthase | - |
| Sus scrofa | Q8MIR4 | bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
| recombinant from Escherichia coli | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| liver | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dephospho-CoA | - |
Homo sapiens | ADP + CoA | - |
? | |
| ATP + dephospho-CoA | - |
Sus scrofa | ADP + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DPCK | - |
Homo sapiens |
| More | bifunctional enzyme is termed CoA synthase | Homo sapiens |
| More | bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 | Homo sapiens |
| More | bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
| 8 | - |
assay at | Sus scrofa |
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