Literature summary for 2.7.1.23 extracted from

Ohashi, K.; Kawai, S.; Koshimizu, M.; Murata, K.
NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme (2011), Mol. Cell. Biochem., 355, 57-64.

Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta-gami(DE3)pLysS cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by NADP+	Homo sapiens
NADH	40% residual activity at 0.5 mM	Homo sapiens
NADPH	66% residual activity at 0.5 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.07	-	NAD+	at pH 8.0 and 50°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	4 * 43000, SDS-PAGE	Homo sapiens
172000	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
TALON column chromatography and Mono Q column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + NAD+	-	Homo sapiens	ADP + NADP+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 43000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
NAD kinase	-	Homo sapiens
NADK	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)