Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | ATP-dependent activity is reduced to 72% in the presence of 20 mM Ca2+ | Corynebacterium glutamicum | |
KCl | 200 mM KCl decreases the polyphosphate-dependent activity to 75% | Corynebacterium glutamicum | |
NaCl | 200 mM NaCl decreases the polyphosphate-dependent activity to 75% | Corynebacterium glutamicum | |
NADH | - |
Corynebacterium glutamicum | |
NADP+ | - |
Corynebacterium glutamicum | |
Ni2+ | ATP-dependent activity is reduced to 60% or 7% in the presence of 5 or 20 mM Ni2+ | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
ATP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.67 | - |
NAD+ | in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
1.1 | - |
NAD+ | in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
2.1 | - |
Tetraphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
2.4 | - |
Triphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
3.1 | - |
polyphosphate(75) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
4.3 | - |
ADP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
5.9 | - |
polyphosphate(20) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
14.1 | - |
polyphosphate(45) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
17.2 | - |
polyphosphate(25) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
18.4 | - |
polyphosphate(65) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme activity is enhanced by Mg2+. The highest activity for ATP is reached with 20 mM Mg2+ (2.6fold higher than with ATP, but without addition of bivalent cations) | Corynebacterium glutamicum | |
Mn2+ | enzyme activity is enhanced by Mn2+. When using polyphosphate, the enzyme requires bivalent cations, preferably manganese ions, for activity. With polyphosphate, the enzyme is most active in the presence of 15 mM Mn2+ (60% activity compared to ATP with 20 mM Mg2+). 20 mM Mn2+ increases ATP-dependent activity 1.9fold | Corynebacterium glutamicum | |
additional information | addition of NaCl or KCl does not alter ATP-dependent activity | Corynebacterium glutamicum | |
additional information | enzyme activity is independent of bivalent cations when using ATP | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34300 | - |
2 * 34300, SDS-PAGE | Corynebacterium glutamicum |
167500 | - |
gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + NAD+ | - |
Corynebacterium glutamicum | AMP + NADP+ | - |
? | |
ADP + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | AMP + NADP+ | - |
? | |
ATP + NAD+ | the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) | Corynebacterium glutamicum | ADP + NADP+ | - |
? | |
ATP + NAD+ | the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) | Corynebacterium glutamicum ATCC 13032 | ADP + NADP+ | - |
? | |
polyphosphate(20) + NAD+ | - |
Corynebacterium glutamicum | polyphosphate(19) + NADP+ | - |
? | |
polyphosphate(20) + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | polyphosphate(19) + NADP+ | - |
? | |
polyphosphate(25) + NAD+ | - |
Corynebacterium glutamicum | polyphosphate(24) + NADP+ | - |
? | |
polyphosphate(25) + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | polyphosphate(24) + NADP+ | - |
? | |
polyphosphate(45) + NAD+ | - |
Corynebacterium glutamicum | polyphosphate(44) + NADP+ | - |
? | |
polyphosphate(45) + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | polyphosphate(44) + NADP+ | - |
? | |
polyphosphate(65) + NAD+ | - |
Corynebacterium glutamicum | polyphosphate(64) + NADP+ | - |
? | |
polyphosphate(75) + NAD+ | - |
Corynebacterium glutamicum | polyphosphate(74) + NADP+ | - |
? | |
tetraphosphate + NAD+ | - |
Corynebacterium glutamicum | triphosphate + NADP+ | - |
? | |
triphosphate + NAD+ | - |
Corynebacterium glutamicum | diphosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 2 * 34300, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
polyP/ATP-dependent NAD kinase | - |
Corynebacterium glutamicum |
Ppnk | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | - |
Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | preheating the enzyme for 1 h at temperatures up to 45°C does not alter the activity. When preheated at 50°C for 1 h, enzyme activity measured at 30°C decreases by 30% and preincubation at 56°C and 60°C leads to a fast and complete loss of activity after 10 and 5 min, respectively | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
polyphosphate(75) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
2.3 | - |
ADP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
3 | - |
Triphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
3.4 | - |
polyphosphate(45) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
3.4 | - |
polyphosphate(65) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
7.3 | - |
polyphosphate(25) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
7.8 | - |
polyphosphate(20) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
7.8 | - |
NAD+ | in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
8.7 | - |
Tetraphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
27.1 | - |
NAD+ | in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
29.2 | - |
ATP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
for polyphosphate-dependent activity | Corynebacterium glutamicum |
7.3 | - |
for ATP-dependent activity | Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NADH | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.34 | - |
NADP+ | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
polyphosphate(65) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.24 | - |
polyphosphate(45) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.42 | - |
polyphosphate(25) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.5 | - |
ADP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
0.58 | - |
polyphosphate(75) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
1.2 | - |
Triphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
1.3 | - |
polyphosphate(20) | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
4.1 | - |
Tetraphosphate | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
7.05 | - |
NAD+ | in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
40.5 | - |
NAD+ | in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum | |
59.6 | - |
ATP | in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C | Corynebacterium glutamicum |