Cloned (Comment) | Organism |
---|---|
- |
Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 6% residual activity | Sphingomonas sp. | |
additional information | not inhibitory: NADH | Sphingomonas sp. | |
NADP+ | 0.1 mM, 38% residual activity | Sphingomonas sp. | |
NADPH | 0.1 mM, 67% residual activity | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
NAD+ | 30°C, pH 8.0 | Sphingomonas sp. | |
1.92 | - |
ATP | 30°C, pH 8.0 | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 46% of activity with MgCl2 | Sphingomonas sp. | |
CoCl2 | 31% of activity with MgCl2 | Sphingomonas sp. | |
MgCl2 | - |
Sphingomonas sp. | |
MnCl2 | 329% of activity with MgCl2 | Sphingomonas sp. | |
ZnCl2 | 231% of activity with MgCl2 | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Sphingomonas sp. |
64000 | - |
gel filtration | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | Q6L7J5 | expression in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.281 | - |
30°C, pH 8.0 | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Sphingomonas sp. | ADP + NADP+ | - |
? | |
CTP + NAD+ | 37% of the activity with ATP | Sphingomonas sp. | CDP + NADP+ | - |
? | |
dATP + NAD+ | 58% of the activity with ATP | Sphingomonas sp. | dADP + NADP+ | - |
? | |
GTP + NAD+ | 100% of the activity with ATP | Sphingomonas sp. | GDP + NADP+ | - |
? | |
TTP + NAD+ | 19% of the activity with ATP | Sphingomonas sp. | TDP + NADP+ | - |
? | |
UTP + NAD+ | 48% of the activity with ATP | Sphingomonas sp. | UDP + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Sphingomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | - |
Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
10 min, 50% residual activity | Sphingomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.95 | - |
NAD+ | 30°C, pH 8.0 | Sphingomonas sp. | |
30.33 | - |
ATP | 30°C, pH 8.0 | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sphingomonas sp. |