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Literature summary for 2.7.1.23 extracted from

  • Ochiai, A.; Mori, S.; Kawai, S.; Murata, K.
    Overexpression, purification, and characterization of ATP-NAD kinase of Sphingomonas sp. A1 (2004), Protein Expr. Purif., 36, 124-130.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Sphingomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, 6% residual activity Sphingomonas sp.
additional information not inhibitory: NADH Sphingomonas sp.
NADP+ 0.1 mM, 38% residual activity Sphingomonas sp.
NADPH 0.1 mM, 67% residual activity Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
NAD+ 30°C, pH 8.0 Sphingomonas sp.
1.92
-
ATP 30°C, pH 8.0 Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 46% of activity with MgCl2 Sphingomonas sp.
CoCl2 31% of activity with MgCl2 Sphingomonas sp.
MgCl2
-
Sphingomonas sp.
MnCl2 329% of activity with MgCl2 Sphingomonas sp.
ZnCl2 231% of activity with MgCl2 Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE Sphingomonas sp.
64000
-
gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. Q6L7J5 expression in Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.281
-
30°C, pH 8.0 Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
Sphingomonas sp. ADP + NADP+
-
?
CTP + NAD+ 37% of the activity with ATP Sphingomonas sp. CDP + NADP+
-
?
dATP + NAD+ 58% of the activity with ATP Sphingomonas sp. dADP + NADP+
-
?
GTP + NAD+ 100% of the activity with ATP Sphingomonas sp. GDP + NADP+
-
?
TTP + NAD+ 19% of the activity with ATP Sphingomonas sp. TDP + NADP+
-
?
UTP + NAD+ 48% of the activity with ATP Sphingomonas sp. UDP + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Sphingomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55
-
Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
10 min, 50% residual activity Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.95
-
NAD+ 30°C, pH 8.0 Sphingomonas sp.
30.33
-
ATP 30°C, pH 8.0 Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sphingomonas sp.