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Literature summary for 2.7.1.23 extracted from

  • Raffaelli, N.; Finaurini, L.; Mazzola, F.; Pucci, L.; Sorci, L.; Amici, A.; Magni, G.
    Characterization of Mycobacterium tuberculosis NAD kinase: functional analysis of the full-length enzyme by site-directed mutagenesis (2004), Biochemistry, 43, 7610-7617.
    View publication on PubMed
Search for more literature for 2.7.1.23

Protein Variants

Protein Variants Comment Organism
G190A no enzymic activity Mycobacterium tuberculosis
G198A no enzymic activity Mycobacterium tuberculosis
G207A no enzymic activity Mycobacterium tuberculosis
G208A decrease in Vmax-value Mycobacterium tuberculosis
L192A no enzymic activity Mycobacterium tuberculosis
P196A no enzymic activity Mycobacterium tuberculosis
S199A decrease in Vmax-value Mycobacterium tuberculosis
T195A kinetic parameters similar to wild-type Mycobacterium tuberculosis
T197A no enzymic activity Mycobacterium tuberculosis
T200A no enzymic activity Mycobacterium tuberculosis
Y202A no enzymic activity Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
NADH 0.4 mM, 43% residual activity Mycobacterium tuberculosis
NADP+ 0.4 mM, 22% residual activity Mycobacterium tuberculosis
NADPH 0.4 mM, 50% residual activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information allosteric enzyme, Hill coefficient for ATP 1.5, for polyphosphate 1.4 Mycobacterium tuberculosis
1.2
-
 NAD+ cosubstrate polyphosphate, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
1.4
-
 NAD+ cosubstrate polyphosphate, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.1
-
 NAD+ cosubstrate polyphosphate, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.2
-
 ATP mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.2
-
 Polyphosphate mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.2
-
 ATP mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.2
-
 Polyphosphate mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.5
-
 ATP wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.5
-
 Polyphosphate wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7
-
 NAD+ cosubstrate ATP, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7
-
 ATP mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7
-
 Polyphosphate mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
3.3
-
 NAD+ cosubstrate ATP, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
3.3
-
 NAD+ cosubstrate ATP, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
3.8
-
 NAD+ cosubstrate polyphosphate, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
6.5
-
 NAD+ cosubstrate ATP, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 or dimer, 3 * 33000, SDS-PAGE Mycobacterium tuberculosis
33000
-
 or trimer, 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
82000
-
 gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 substrates ATP, NAD+, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
0.7
-
 substrates ATP, NAD+, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
1.1
-
 substrates ATP, NAD+, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
1.2
-
 substrates ATP, NAD+, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.2
-
 substrates polyphosphate, NAD+, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.5
-
 substrates polyphosphate, NAD+, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
3.5
-
 substrates polyphosphate, NAD+, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
3.8
-
 substrates polyphosphate, NAD+, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-20°C, stable for several weeks Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Mycobacterium tuberculosis ADP + NADP+
-
 ?
polyphosphate + NAD+
-
 Mycobacterium tuberculosis (phosphate)n-1 + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer or trimer, 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
trimer or dimer, 3 * 33000, SDS-PAGE Mycobacterium tuberculosis