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Literature summary for 2.7.1.23 extracted from

  • Gallais, S.; de Crescenzo, M.A.; Laval-Martin, D.L.
    Characterization of soluble calcium calmodulin-dependent and -independent NAD+ kinases from Avena sativa seeds (2001), Aust. J. Plant Physiol., 28, 363-371.
No PubMed abstract available
Search for more literature for 2.7.1.23

Activating Compound

Activating Compound Comment Organism Structure
adenine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
AMP 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
Caffeine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
Calmodulin a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa
D-ribose 5-phosphate 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
imidazole 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
nicotinic acid 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
Pyridine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa

General Stability

General Stability Organism
partial loss of activity after freezing and thawing Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
ADP 5 mM, 4% inhibition of the 410000 Da isoform, 25% inhibition of the 63000 Da isoform Avena sativa
chlorpromazin 5 mM, 11% inhibition of the 410000 Da isoform, 80% inhibition of the 63000 Da isoform Avena sativa
diphosphate 5 mM, 46% inhibition of the 410000 Da isoform, 69% inhibition of the 63000 Da isoform Avena sativa
EGTA 5 mM, 20% inhibition of the 410000 Da isoform, 85% inhibition of the 63000 Da isoform Avena sativa
iodoacetic acid 1 mM, 56% inhibition of the 63000 Da isoform, no inhibition of the 410000 Da isoform Avena sativa
NEM 1 mM, 92% inhibition of the 63000 Da isoform, 34% inhibition of the 410000 Da isoform Avena sativa
R24571 1 mM, 46% inhibition of the 410000 Da isoform, 89% inhibition of the 63000 da isoform Avena sativa
Trifluoperazine 2 mM, 8% inhibition of the 410000 da isoform, 83% inhibition of the 63000 Da isoform Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 Ca2+-calmodulin-dependent isoenzyme Avena sativa
410000
-
 Ca2+-calmodulin-independent NAD+ kinase, gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NAD+ Avena sativa the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways ADP + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed culture of after-ripened and dormant seeds Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0009966
-
-
 Avena sativa

Storage Stability

Storage Stability Organism
-80°C, 30% v/v glycerol, stable for more than 3 months Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+ the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways Avena sativa ADP + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 410000 Da Ca2+-calmodulin-independent isoform and 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa

pH Range

pH Minimum pH Maximum Comment Organism
6.8 9.2 90% of maximal activity between pH 6.8 and pH 9.2, 410000 Da Ca2+-calmodulin-independent isoform Avena sativa
7.2 8.5 90% of maximal activity between pH 7.2 and pH 8.5, 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa