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Literature summary for 2.7.1.23 extracted from

  • Kawai, S.; Mori, S.; Mukai, T.; Hashimoto, W.; Murata, K.
    Molecular characterization of Escherichia coli NAD kinase (2001), Eur. J. Biochem., 268, 4359-4365.
    View publication on PubMed
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 0.25 mM, 65% inhibition Escherichia coli
NADH 0.01 mM, 61% inhibition Escherichia coli
NADP+ 0.1 mM, 21% inhibition Escherichia coli
NADPH 0.01 mM, 76% inhibition Escherichia coli
PCMB 0.25 mM, 67% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 NAD+ pH 7.0, 37°C Escherichia coli
2.5
-
 ATP pH 7.0, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 6 * 30000, SDS-PAGE Escherichia coli
180000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 MG1655
-
Escherichia coli MG1655
-
 MG1655
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Escherichia coli ADP + NADP+
-
 ?
ATP + NAD+
-
 Escherichia coli MG1655 ADP + NADP+
-
 ?
CTP + NAD+ 60% of activity with ATP Escherichia coli CDP + NADP+
-
 ?
CTP + NAD+ 60% of activity with ATP Escherichia coli MG1655 CDP + NADP+
-
 ?
dATP + NAD+ 42% of the activity with ATP Escherichia coli dADP + NADP+
-
 ?
dATP + NAD+ 42% of the activity with ATP Escherichia coli MG1655 dADP + NADP+
-
 ?
GTP + NAD+ 56% of activity with ATP Escherichia coli GDP + NADP+
-
 ?
GTP + NAD+ 56% of activity with ATP Escherichia coli MG1655 GDP + NADP+
-
 ?
TTP + NAD+ 40% of maximal activity Escherichia coli TDP + NADP+
-
 ?
UTP + NAD+ 109% of the activity with ATP Escherichia coli UDP + NADP+
-
 ?
UTP + NAD+ 109% of the activity with ATP Escherichia coli MG1655 UDP + NADP+
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 68 about 50% of maximal activity at 37°C and at 68°C Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable Escherichia coli
60
-
 10 min, about 10% loss of activity Escherichia coli
65
-
 10 min, about 45% loss of activity Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55
-
 ATP pH 7.0, 37°C Escherichia coli
125
-
 NAD+ pH 7.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Tris/HCl buffer Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 40% of maximal activity, pH 10.0: about 80% of maximal activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli