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Literature summary for 2.7.1.23 extracted from

  • Ji, X.; Li, H.; Yuan, Z.; Liu, S.
    Immobilization of NAD kinase (1984), Ann. N. Y. Acad. Sci., 434, 264-266.
    View publication on PubMed
Search for more literature for 2.7.1.23

General Stability

General Stability Organism
stability is enhanced by immobilization on CNBr-activated Sepharose 4B Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 NAD+ native enzyme Gallus gallus
1.5
-
 NAD+ enzyme immobilized on CNBr-activated Sepharose 4B Gallus gallus
2.5
-
 ATP native enzyme Gallus gallus
4.5
-
 ATP enzyme immobilized on CNBr-activated Sepharose 4B Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Storage Stability

Storage Stability Organism
4°C, 0.1 M phosphate buffer, pH 7.5, activity of the native enzyme is entirely lost after 55 days, enzyme immobilized and CNBr-activated Sepharose 4B retains activity without any detectable loss of after 70 days Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Gallus gallus ADP + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 native enzyme Gallus gallus
55
-
 enzyme immobilized on CNBr-activated Sepharose 4B Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme immobilized on CNBr-activated Sepharose is more stable against heat than the native enzyme Gallus gallus
40
-
 native enzyme and immobilized enzyme, stable Gallus gallus
55
-
 immobilized enzyme is more stable than the native enzyme above 55°C Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 enzyme immobilized on CNBr-activated Sepharose 4B, phosphate buffer Gallus gallus
7
-
 native enzyme, phosphate buffer, Tris-HCl buffer Gallus gallus
7.5
-
 enzyme immobilized on CNBr-activated Sepharose 4B, Tris-HCl buffer Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 37°C, 2 h, native and immobilized stable Gallus gallus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gallus gallus