Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3',5'-cAMP | 3.5 mM, 20% inhibition | Strongylocentrotus purpuratus | |
adenosine diphosphoribose | 3.5 mM, 31% inhibition | Strongylocentrotus purpuratus | |
ADP | 3.5 mM, 63% inhibition | Strongylocentrotus purpuratus | |
AMP | 3.5 mM, 29% inhibition | Strongylocentrotus purpuratus | |
citrate | 3.5 mM, 55% inhibition | Strongylocentrotus purpuratus | |
Mn2+ | above 1 mM | Strongylocentrotus purpuratus | |
NADH | - |
Strongylocentrotus purpuratus | |
NADPH | - |
Strongylocentrotus purpuratus | |
phosphoenolpyruvate | 3.5 mM, 20% inhibition | Strongylocentrotus purpuratus | |
pyruvate | 3.5 mM, 10% inhibition | Strongylocentrotus purpuratus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 33% of the activation obtained with Mn2+ | Strongylocentrotus purpuratus | |
Mg2+ | divalent cation required | Strongylocentrotus purpuratus | |
Mg2+ | maximal activation at a Mg2+:ATP ratio of 1:1 | Strongylocentrotus purpuratus | |
Mn2+ | divalent cation required | Strongylocentrotus purpuratus | |
Mn2+ | at pH 6.5, with 2.0 mM NAD+ and 1.0 mM ATP maximal activity is observed with Mn2+ at 0.5 to 1.0 mM | Strongylocentrotus purpuratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Strongylocentrotus purpuratus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Strongylocentrotus purpuratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Strongylocentrotus purpuratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.222 | - |
- |
Strongylocentrotus purpuratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Strongylocentrotus purpuratus | ADP + NADP+ | - |
? | |
CTP + NAD+ | 11% of the activity with ATP | Strongylocentrotus purpuratus | CDP + NADP+ | - |
? | |
GTP + NAD+ | 14% of the activity with ATP | Strongylocentrotus purpuratus | GDP + NADP+ | - |
? | |
ITP + NAD+ | 7% of the activity with ATP | Strongylocentrotus purpuratus | IDP + NADP+ | - |
? | |
UTP + NAD+ | 5% of the activity with ATP | Strongylocentrotus purpuratus | UDP + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Strongylocentrotus purpuratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | about 50% of maximal activity at pH 5.5 and pH 7.5 | Strongylocentrotus purpuratus |
6 | 7 | activity decreases sharply below pH 6.0 and above pH 7.0 | Strongylocentrotus purpuratus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
4°C, 18 h, about 60% loss of activity, irreversible loss of activity | Strongylocentrotus purpuratus |
6 | 7 | 4°C, 18 h, stable | Strongylocentrotus purpuratus |
8 | - |
4°C, 18 h, about 50% loss of activity, loss of activity is reversible by dialysis to pH 6.2 | Strongylocentrotus purpuratus |
8.5 | - |
4°C, 18 h, about 70% loss of activity, loss of activity is reversible by dialysis to pH 6.2 | Strongylocentrotus purpuratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Strongylocentrotus purpuratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | pH 6.5, 30°C | Strongylocentrotus purpuratus | |
0.03 | - |
NADH | pH 6.5, 30°C | Strongylocentrotus purpuratus |