Literature summary for 2.7.1.207 extracted from

Lessard, C.; Cochu, A.; Lemay, J.D.; Roy, D.; Vaillancourt, K.; Frenette, M.; Moineau, S.; Vadeboncoeur, C.
Phosphorylation of Streptococcus salivarius lactose permease (LacS) by HPr(His - P) and HPr(Ser-P)(His - P) and effects on growth (2003), J. Bacteriol., 185, 6764-6772.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptococcus salivarius

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Streptococcus salivarius	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1]	Streptococcus salivarius	-	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + lactose[side 1]	Streptococcus salivarius ATCC 25975	-	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus salivarius	-	-	-
Streptococcus salivarius ATCC 25975	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	domain IIALacS is reversibly phosphorylated on a histidine residue at position 552	Streptococcus salivarius

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and MonoQ HR column chromatography	Streptococcus salivarius

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1]	-	Streptococcus salivarius	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + lactose[side 1]	-	Streptococcus salivarius ATCC 25975	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
IIALacS	domain	Streptococcus salivarius
LACS	-	Streptococcus salivarius
lactose permease	-	Streptococcus salivarius

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Release 2023.1 (January 2023)