Literature summary for 2.7.1.20 extracted from

Datta, R.; Das, I.; Sen, B.; Chakraborty, A.; Adak, S.; Mandal, C.; Datta, A.K.
Homology-model-guided site-specific mutagenesis reveals the mechanisms of substrate binding and product-regulation of adenosine kinase from Leishmania donovani (2006), Biochem. J., 394, 35-42.

Search for more literature for 2.7.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Leishmania donovani

Protein Variants

Protein Variants	Comment	Organism
D16E	specific activity at the highest attainable adenosine concentration is approximately 2.5-fold lower than that of the wild-type enzyme. Non-saturation kinetics of the D16E mutant are indicative of weaker binding	Leishmania donovani
D16N	kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP	Leishmania donovani
D16V	kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP	Leishmania donovani
R131A	mutant shows abrogated AMP-binding, insensitivity towards AMP inhibition, unaltered Km-value for adenosine	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
2'-deoxyadenosine	-	Leishmania donovani
3'-deoxyadenosine	-	Leishmania donovani
AMP	inhibits both D16V and D16N mutant enzymes to similar extents to that observed with the wild-type enzyme, displays a much reduced ability in inhibiting R131A mutant enzyme	Leishmania donovani

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	adenosine	wild-type enzyme	Leishmania donovani
0.0059	-	adenosine	mutant enzyme R131A	Leishmania donovani
0.017	-	ATP	mutant enzyme D16N	Leishmania donovani
0.02	-	ATP	mutant enzyme D16V	Leishmania donovani
0.036	-	ATP	wild-type enzyme	Leishmania donovani
0.036	-	3'-deoxyadenosine	wild-type enzyme	Leishmania donovani
0.05	-	ATP	mutant enzyme R131A	Leishmania donovani
0.077	-	2'-deoxyadenosine	wild-type enzyme	Leishmania donovani
1.293	-	adenosine	mutant enzyme D16V	Leishmania donovani
1.575	-	adenosine	mutant enzyme D16N	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2'-deoxy-adenosine	-	Leishmania donovani	ADP + 2'-deoxy-AMP	-	?
ATP + adenosine	-	Leishmania donovani	ADP + AMP	-	?
ATP + cordycepin	-	Leishmania donovani	ADP + cordycepin 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
LdAdK	-	Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.733	-	ATP	mutant enzyme R131A	Leishmania donovani
0.733	-	adenosine	mutant enzyme R131A	Leishmania donovani
5.1	-	ATP	mutant enzyme D16V	Leishmania donovani
5.1	-	adenosine	mutant enzyme D16V	Leishmania donovani
5.2	-	ATP	mutant enzyme D16N	Leishmania donovani
5.2	-	adenosine	mutant enzyme D16N	Leishmania donovani
5.25	-	2'-deoxyadenosine	wild-type enzyme	Leishmania donovani
6.7	-	3'-deoxyadenosine	wild-type enzyme	Leishmania donovani
18	-	ATP	wild-type enzyme	Leishmania donovani
18	-	adenosine	wild-type enzyme	Leishmania donovani
24.15	-	ATP	wild-type enzyme	Leishmania donovani
24.15	-	adenosine	wild-type enzyme	Leishmania donovani

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0033	-	3'-deoxyadenosine	wild-type enzyme	Leishmania donovani
0.01575	-	2'-deoxyadenosine	wild-type enzyme	Leishmania donovani

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)