Cloned (Comment) | Organism |
---|---|
expression of genes nanK, yajF, and ycfX in glk-deficient mutant strain BM5340(DE3) | Escherichia coli K-12 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme-deficient glk mutant strain BM5340(DE3), the mutant strain can be complemented by expression of Escherichia coli genes nanK, yajF, and ycfX, which express proteins with 4fold lower glucokinase activity and ambiguous substrate specificities, overview | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
D-glucose | recombinant Glk, pH 7.6, 25°C | Escherichia coli K-12 | |
0.26 | - |
ATP | recombinant Glk, pH 7.6, 25°C | Escherichia coli K-12 | |
0.31 | - |
D-glucose | recombinant YajF, pH 7.6, 25°C | Escherichia coli K-12 | |
0.51 | - |
ATP | recombinant YajF, pH 7.6, 25°C | Escherichia coli K-12 | |
2.9 | - |
ATP | recombinant NanK, pH 7.6, 25°C | Escherichia coli K-12 | |
3.4 | - |
ATP | recombinant YcfX, pH 7.6, 25°C | Escherichia coli K-12 | |
3.8 | - |
D-glucose | recombinant YcfX, pH 7.6, 25°C | Escherichia coli K-12 | |
18 | - |
D-glucose | recombinant NanK, pH 7.6, 25°C | Escherichia coli K-12 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli K-12 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Escherichia coli K-12 | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
several strains | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Escherichia coli K-12 | ADP + D-glucose 6-phosphate | - |
? | |
additional information | recombinant enzymes NanK and YajF also utilize N-acetyl-D-mannosamine and D-fructose as substrates, respectively | Escherichia coli K-12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glk | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
D-glucose | recombinant NanK, pH 7.6, 25°C | Escherichia coli K-12 | |
9.2 | - |
D-glucose | recombinant YcfX, pH 7.6, 25°C | Escherichia coli K-12 | |
12 | - |
D-glucose | recombinant Glk, pH 7.6, 25°C | Escherichia coli K-12 | |
410 | - |
D-glucose | recombinant Glk, pH 7.6, 25°C | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli K-12 |