Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
D-glucose | 50°C, pH 8 | Aeropyrum pernix | |
0.5 | - |
ATP | 50°C, pH 8 | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Aeropyrum pernix | |
Mg2+ | - |
Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Aeropyrum pernix | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.2 | - |
recombinant protein | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | no reaction with the recombinant enzyme | Aeropyrum pernix | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-fructose | no reaction with the recombinant enzyme | Aeropyrum pernix | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucosamine | no reaction with the recombinant enzyme | Aeropyrum pernix | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucose | - |
Aeropyrum pernix | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | no reaction with the recombinant enzyme | Aeropyrum pernix | ADP + D-mannose 6-phosphate | - |
? | |
GTP + D-glucose | no reaction with the recombinant enzyme | Aeropyrum pernix | GDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | - |
Aeropyrum pernix | IDP + D-glucose 6-phosphate | - |
? | |
UTP + D-glucose | no reaction with the recombinant enzyme | Aeropyrum pernix | UDP + D-glucose 6-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
full activity after 10 min | Aeropyrum pernix |
100 | - |
retains 65% of activity after 10 min | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aeropyrum pernix |