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Literature summary for 2.7.1.2 extracted from

  • Klein, D.P.; Charles, A.M.
    Purification and physical properties of glucokinase from Thiobacillus versutus (A2) (1986), Can. J. Microbiol., 32, 937-941.
No PubMed abstract available
Search for more literature for 2.7.1.2

General Stability

General Stability Organism
glucose or DTT protects against thermal inactivation at 25°C Paracoccus versutus

Inhibitors

Inhibitors Comment Organism Structure
N-acetylglucosamine weak Paracoccus versutus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
 ATP
-
 Paracoccus versutus
0.86
-
 D-glucose
-
 Paracoccus versutus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can partially replace Mn2+ in activation Paracoccus versutus
Co2+ 38.5% of the activity with Mn2+ Paracoccus versutus
Mg2+ Km: 0.27 mM Paracoccus versutus
Mg2+ absolute requirement for divalent cation, 78% of the activity with Mn2+ Paracoccus versutus
Mn2+ absolute requirement for divalent cation, Mn2+ most effective Paracoccus versutus
Ni2+ can partially replace Mn2+ in activation Paracoccus versutus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67500
-
 density gradient centrifugation Paracoccus versutus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Paracoccus versutus
-
 ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose Paracoccus versutus A2
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Paracoccus versutus
-
 A2
-
Paracoccus versutus A2
-
 A2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paracoccus versutus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
74.9
-
-
 Paracoccus versutus

Storage Stability

Storage Stability Organism
-20°C, stable for 4 weeks Paracoccus versutus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Paracoccus versutus ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose ATP is the most efficient phosphoryl donor Paracoccus versutus ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Paracoccus versutus A2 ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose ATP is the most efficient phosphoryl donor Paracoccus versutus A2 ADP + D-glucose 6-phosphate
-
 ?
CTP + D-glucose
-
 Paracoccus versutus CDP + D-glucose 6-phosphate
-
 ?
CTP + D-glucose
-
 Paracoccus versutus A2 CDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose 30% of the activity with ATP Paracoccus versutus IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose 30% of the activity with ATP Paracoccus versutus A2 IDP + D-glucose 6-phosphate
-
 ?
additional information less than 10% of the activity with ATP: TTP Paracoccus versutus ?
-
 ?
additional information less than 10% of the activity with ATP: TTP Paracoccus versutus A2 ?
-
 ?
UTP + D-glucose 12% of the activity with ATP Paracoccus versutus UDP + D-glucose 6-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25
-
 Paracoccus versutus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 glucose or DTT protects against thermal inactivation at 25°C Paracoccus versutus
25
-
 48 h, with glucose and DTT stable, unstable without Paracoccus versutus
55
-
 1 min, complete loss of activity Paracoccus versutus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Paracoccus versutus