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Literature summary for 2.7.1.2 extracted from
- Isolation and characterization of a thermophilic glucokinase from Bacillus stearothermophilus (1973), FEBS Lett., 37, 212-216.
General Stability
| General Stability | Organism |
|---|---|
| glucose increases thermal stability | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
2 * 34500, SDS-PAGE | Geobacillus stearothermophilus |
| 67000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Geobacillus stearothermophilus | - |
ADP + D-glucose 6-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 304 | - |
- |
Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, 1 mM DTT, 5 mM MgCl2, stable for 1 week , 4°C, suspended precipitate in a 60% saturated solution of ammonium sulfate, stable for at least 3 months | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | highly specific for D-glucose | Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
| CTP + D-glucose | 10% of the activity with ATP | Geobacillus stearothermophilus | CDP + D-glucose 6-phosphate | - |
? | |
| GTP + D-glucose | 30% of the activity with ATP | Geobacillus stearothermophilus | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | 75% of the activity with ATP | Geobacillus stearothermophilus | IDP + D-glucose 6-phosphate | - |
? | |
| UTP + D-glucose | 32% of the activity with ATP | Geobacillus stearothermophilus | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 34500, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glucose increases thermal stability | Geobacillus stearothermophilus |
| 60 | - |
no loss of activity in 30 min | Geobacillus stearothermophilus |
| 70 | - |
about 10% loss of activity in 30 min, presence of 0.01 M glucose + 0.2 M NaCl | Geobacillus stearothermophilus |
| 75 | - |
about 25% loss of activity in 30 min, presence of 0.01 M glucose + 0.2 M NaCl, about 90% loss of activity, presence of 0.01 M glucose absence of NaCl | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Geobacillus stearothermophilus |
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