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Literature summary for 2.7.1.197 extracted from
- Evidence for two distinct conformations of the Escherichia coli mannitol permease that are important for its transport and phosphorylation functions (1989), J. Cell. Biochem., 39, 207-216.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | inorganic phosphate increases the Vmax, of the transphosphorylation reaction 4.5-fold and increases Km for both substrates approximately 2fold | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.124 | - |
D-mannitol 1-phosphate[side 2] | in HEPES buffer, at pH 7.0 and 37°C | Escherichia coli | |
| 0.29 | - |
D-mannitol[side 1] | in HEPES buffer, at pH 7.0 and 37°C | Escherichia coli | |
| 0.29 | - |
D-mannitol 1-phosphate[side 2] | in sodium phosphate buffer, at pH 7.0 and 37°C | Escherichia coli | |
| 0.46 | - |
D-mannitol[side 1] | in sodium phosphate buffer, at pH 7.0 and 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Escherichia coli |
| 68000 | - |
2 * 68000, calculated from amino acid sequence | Escherichia coli |
| 130000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [heat-stable phosphocarrier protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | - |
[heat-stable phosphocarrier protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
| [heat-stable phosphocarrier protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli KL141 | - |
[heat-stable phosphocarrier protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli KL141 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-200 gel filtration | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [heat-stable phosphocarrier protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli | [heat-stable phosphocarrier protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
| [heat-stable phosphocarrier protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli KL141 | [heat-stable phosphocarrier protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Escherichia coli |
| dimer | 2 * 68000, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannitol permease | - |
Escherichia coli |
| mannitolspecific enzyme II of the phosphotransferase system | - |
Escherichia coli |
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