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Literature summary for 2.7.1.197 extracted from
- Substrate and phospholipid specificity of the purified mannitol permease of Escherichia coli (1983), J. Cell. Biochem., 23, 231-240.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | - |
Escherichia coli |  |
| phosphatidylethanolamine | - |
Escherichia coli | |
| phosphatidylglycerol | - |
Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-amino-2-deoxy-D-mannitol | 63% residual activity at 2 mM | Escherichia coli | |
| 2-deoxy-D-mannitol | 73% residual activity at 2 mM; 74% residual activity at 2 mM | Escherichia coli | |
| D-arabitol | - |
Escherichia coli | |
| D-glucitol | 73% residual activity at 2 mM | Escherichia coli | |
| D-mannoheptitol | 9% residual activity at 2 mM | Escherichia coli | |
| D-mannonic acid | - |
Escherichia coli | |
| additional information | 1,6-dibromo-1,6-dideoxy-D-mannitol, D,L-threitol, L-threitol, erythritol, and glycerol do not inhibit the enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
D-mannitol[side 1] | at pH 8.0 and 37°C | Escherichia coli | |
| 0.2 | - |
2-amino-2-deoxy-D-mannitol[side 1] | at pH 8.0 and 37°C | Escherichia coli | |
| 0.5 | - |
2-deoxy-D-mannitol[side 1] | at pH 8.0 and 37°C | Escherichia coli | |
| 0.5 | - |
D-arabitol[side 1] | Km above 0.5 mM, at pH 8.0 and 37°C | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | the enzyme is highly, but not absolutely, specific for D-mannitol | [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00550 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with D-galactitol, D-ribitol, and D-xylitol | Escherichia coli | ? | - |
? | |
| [protein]-Npi-phospho-L-histidine + 2-amino-2-deoxy-D-mannitol[side 1] | 50% activity compared to D-mannitol | Escherichia coli | [protein]-L-histidine + 2-amino-2-deoxy-D-mannitol 1-phosphate[side 2] | - |
? | |
| [protein]-Npi-phospho-L-histidine + 2-deoxy-D-mannitol[side 1] | 32% activity compared to D-mannitol | Escherichia coli | [protein]-L-histidine + 2-deoxy-D-mannitol 1-phosphate[side 2] | - |
? | |
| [protein]-Npi-phospho-L-histidine + D-arabitol[side 1] | 11% activity compared to D-mannitol | Escherichia coli | [protein]-L-histidine + D-arabitol 1-phosphate[side 2] | - |
? | |
| [protein]-Npi-phospho-L-histidine + D-glucitol[side 1] | 37% activity compared to D-mannitol | Escherichia coli | [protein]-L-histidine + D-glucitol 1-phosphate[side 2] | - |
? | |
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | the enzyme is highly, but not absolutely, specific for D-mannitol | Escherichia coli | [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-rnannitol-specific enzyme I1 | - |
Escherichia coli |
| mannitol permease | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
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