Literature summary for 2.7.1.197 extracted from

Jacobson, G.; Saraceni-Richards, C.
The Escherichia coli mannitol permease as a model for transport via the bacterial phosphotransferase system (1993), J. Bioenerg. Biomembr., 25, 621-626.

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Protein Variants

Protein Variants	Comment	Organism
E218A	the mutant has a high Km for mannitol but can carry out facilitated diffusion of mannitol at a rate higher than the wild type protein in its unphosphorylated form	Escherichia coli
E257A	the mutant does not detectably bind mannitol up to a concentration of at least 1 mM	Escherichia coli
G253E	the mutant cannot transport but still can phosphorylate mannitol	Escherichia coli
H195A	the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations	Escherichia coli
H195R	the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1]	Escherichia coli	-	[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00550	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1]	-	Escherichia coli	[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
mannitol permease	the enzyme consists of two cytoplasmic domains (EIIA and EIIB) involved in mannitol phosphorylation and an integral membrane domain (EIIC) which is sufficient to bind mannitol, but which transports mannitol at a rate that is dependent on phosphorylation of the EIIA and EIIB domains	Escherichia coli

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Release 2023.1 (January 2023)