Literature summary for 2.7.1.190 extracted from

Smith, C.A.; Toth, M.; Frase, H.; Byrnes, L.J.; Vakulenko, S.B.
Aminoglycoside 2-phosphotransferase IIIa (APH(2)-IIIa) prefers GTP over ATP: structural templates for nucleotide recognition in the bacterial aminoglycoside-2 kinases (2012), J. Biol. Chem., 287, 12893-12903.

Search for more literature for 2.7.1.190

Cloned(Commentary)

Cloned (Comment)	Organism
-	Enterococcus gallinarum

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.2 A resolution. Access to the ATP-binding template is blocked by a bulky tyrosine residue. Substitution of this tyrosine by a smaller amino acid opens access to the ATP template	Enterococcus gallinarum

Protein Variants

Protein Variants	Comment	Organism
Y92A	residue Y92 blocks access to the ATP-binding template. Mutant shows 8fold decrease in km value for ATP	Enterococcus gallinarum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	GTP	wild-type, pH not specified in the publication, temperature not specified in the publication	Enterococcus gallinarum
0.094	-	GTP	mutant Y92A, pH not specified in the publication, temperature not specified in the publication	Enterococcus gallinarum
0.22	-	ATP	mutant Y92A, pH not specified in the publication, temperature not specified in the publication	Enterococcus gallinarum
1.6	-	ATP	wild-type, pH not specified in the publication, temperature not specified in the publication	Enterococcus gallinarum

Organism

Organism	UniProt	Comment	Textmining
Enterococcus gallinarum	P96762	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + kanamycin	-	Enterococcus gallinarum	ADP + kanamycin 2''-phosphate	-	?
GTP + kanamycin	-	Enterococcus gallinarum	GDP + kanamycin 2''-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
gentamicin resistance protein	-	Enterococcus gallinarum

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Release 2023.1 (January 2023)