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Literature summary for 2.7.1.190 extracted from

  • Badarau, A.; Shi, Q.; Chow, J.W.; Zajicek, J.; Mobashery, S.; Vakulenko, S.
    Aminoglycoside 2''-phosphotransferase type IIIa from Enterococcus (2008), J. Biol. Chem., 283, 7638-7647.
    View publication on PubMed
Search for more literature for 2.7.1.190

Inhibitors

Inhibitors Comment Organism Structure
apramycin
-
 Enterococcus sp.
butirosin
-
 Enterococcus sp.
lividomycin A
-
 Enterococcus sp.
neamine
-
 Enterococcus sp.
neomycin
-
 Enterococcus sp.
paromomycin
-
 Enterococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
0.0045
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
0.039
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
0.09
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
0.1
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.

Organism

Organism UniProt Comment Textmining
Enterococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + kanamycin A
-
 Enterococcus sp. ADP + kanamycin A 2''-phosphate
-
 ?
GTP + gentamicin C
-
 Enterococcus sp. GDP + gentamicin C 2''-phosphate GTP is preferred over ATP ?
GTP + netilmicin
-
 Enterococcus sp. GDP + netilmicin 2''-phosphate
-
 ?
ITP + dibekacin
-
 Enterococcus sp. IDP + dibekacin 2''-phosphate
-
 ?
additional information random sequential Bi Bi mechanism. At pH 7.5 the release of guanosine triphosphate is rate-limiting. No substrates: amikacin, isepamicin, neomycin, butirosin, lividomycin A , paromomycin, spectinomycin, streptomycin, apramycin, hygromycin, neamine Enterococcus sp. ?
-
 ?
UTP + tobramycin
-
 Enterococcus sp. UDP + tobramycin 2''-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
aminoglycoside 2''-phosphotransferase type IIIa
-
 Enterococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
0.14
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
0.5
-
 gentamicin C pH 7.5, 25°C Enterococcus sp.
0.7
-
 tobramycin pH 7.5, 25°C Enterococcus sp.
0.7
-
 netilmicin pH 7.5, 25°C Enterococcus sp.
0.7
-
 dibekacin pH 7.5, 25°C Enterococcus sp.
0.8
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
0.8
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.1
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
1.4
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
56
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
180
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
180
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
670
-
 netilmicin pH 7.5, 25°C Enterococcus sp.
1800
-
 gentamicin C pH 7.5, 25°C Enterococcus sp.
2200
-
 tobramycin pH 7.5, 25°C Enterococcus sp.
2300
-
 dibekacin pH 7.5, 25°C Enterococcus sp.