Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
H100A | reduced activity | Cereibacter sphaeroides |
H100N | reduced activity | Cereibacter sphaeroides |
H100Q | reduced activity | Cereibacter sphaeroides |
H45N | similar to wild type enzyme | Cereibacter sphaeroides |
Y98L | reduced activity | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-phosphogluconate | competitive inhibitor, 78% residual activity at 1 mM | Cereibacter sphaeroides | |
diethyl dicarbonate | 27% residual activity at 0.04 mM | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-ribulose 5-phosphate | wild type enzyme, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate | Cereibacter sphaeroides | |
0.78 | - |
D-ribulose 5-phosphate | mutant enzyme H100A, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate | Cereibacter sphaeroides | |
1.35 | - |
D-ribulose 5-phosphate | mutant enzyme H100Q, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate | Cereibacter sphaeroides | |
1.37 | - |
D-ribulose 5-phosphate | mutant enzyme H100N, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate | Cereibacter sphaeroides | |
4 | - |
D-ribulose 5-phosphate | mutant enzyme Y98L, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose chromatography | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + ATP | - |
Cereibacter sphaeroides | D-ribulose 1,5-diphosphate + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Prk | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cereibacter sphaeroides |