Literature summary for 2.7.1.189 extracted from

Zargar, A.; Quan, D.N.; Emamian, M.; Tsao, C.Y.; Wu, H.C.; Virgile, C.R.; Bentley, W.E.
Rational design of controller cells to manipulate protein and phenotype expression (2015), Metab. Eng., 30, 61-68.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene lsrK, overexpression in Escherichia coli strain LW12	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Escherichia coli	5622	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + autoinducer-2	Escherichia coli	i.e. AI-2	ADP + phosphorylated autoinducer-2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	W3110, gene lsrK	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + autoinducer-2	i.e. AI-2	Escherichia coli	ADP + phosphorylated autoinducer-2	-	?

Synonyms

Synonyms	Comment	Organism
AI-2 kinase	-	Escherichia coli
Lsr kinase	-	Escherichia coli
lsrK	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

General Information

General Information	Comment	Organism
metabolism	model-based design, construction, and characterization of controller cells to modulate the external AI-2 environment, the cells are designed via the compartmentalization of different aspects of AI-2 processing: uptake (lsrACDB), phosphorylation (lsrK), and degradation (lsrFG), mathematical model overview	Escherichia coli
physiological function	the enzyme is responsible for the phosphorylation of autoinducer 2, and thereby attenuating cell-cell communication among bacterial populations. The bacterial signal molecule autoinducer-2 is secreted as a quorum sensing signal. The enzyme LsrK increases the net uptake rate by limiting secretion of AI-2 back into the extracellular environment. LsrK-mediated phosphorylation of AI-2 blocks export back to the extracellular milieu so that accumulated AI-2P binds the regulatory protein LsrR	Escherichia coli

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Release 2023.1 (January 2023)