Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.189 extracted from

  • Xavier, K.B.; Miller, S.T.; Lu, W.; Kim, J.H.; Rabinowitz, J.; Pelczer, I.; Semmelhack, M.F.; Bassler, B.L.
    Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria (2007), ACS Chem. Biol., 2, 128-136.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lsrK, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4,5-dihydroxypentane-2,3-dione Salmonella enterica the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + 4,5-dihydroxypentane-2,3-dione Salmonella enterica serovar Typhimurium the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + autoinducer-2 Salmonella enterica
-
ADP + phosphorylated autoinducer-2
-
?
ATP + autoinducer-2 Salmonella enterica serovar Typhimurium
-
ADP + phosphorylated autoinducer-2
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
serovar Typhimurium, gene lsrK
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography and gel filtration Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 Salmonella enterica ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, mass spectrometric product identification. Molecule (S)-4,5-dihydroxypentane-2,3-dione (DPD) is the precursor of the signal molecule autoinducer-2 Salmonella enterica ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 Salmonella enterica serovar Typhimurium ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, mass spectrometric product identification. Molecule (S)-4,5-dihydroxypentane-2,3-dione (DPD) is the precursor of the signal molecule autoinducer-2 Salmonella enterica serovar Typhimurium ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
?
ATP + autoinducer-2
-
Salmonella enterica ADP + phosphorylated autoinducer-2
-
?
ATP + autoinducer-2 i.e. AI-2 Salmonella enterica ADP + phosphorylated autoinducer-2
-
?
ATP + autoinducer-2
-
Salmonella enterica serovar Typhimurium ADP + phosphorylated autoinducer-2
-
?

Synonyms

Synonyms Comment Organism
lsrK
-
Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
ATP
-
Salmonella enterica

Expression

Organism Comment Expression
Salmonella enterica phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon up

General Information

General Information Comment Organism
physiological function enteric bacteria respond to the presence of competitor bacteria by sequestering and destroying autoinducer-2, through phosphorylation by the enzyme LsrK and cleavage of 4-hydroxy-2,3-pentanedione 5-phosphate producing 2-phosphoglycolic acid via enzyme LsrG, thereby eliminating the competitors' intercellular communication capabilities. The phosphorylated form of AI-2 is the intracellular signal responsible for lsr activation Salmonella enterica