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Literature summary for 2.7.1.185 extracted from

  • Vinokur, J.M.; Korman, T.P.; Cao, Z.; Bowie, J.U.
    Evidence of a novel mevalonate pathway in archaea (2014), Biochemistry, 53, 4161-4168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.185

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Thermoplasma acidophilum mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HIN1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
 Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?
ATP + (R)-mevalonate mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?

Synonyms

Synonyms Comment Organism
Ta1305 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermoplasma acidophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: about 55% of maximal activity, 60°C: about 95% of maximal activity Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, enzyme retains more than 95% of its activity Thermoplasma acidophilum
70
-
 1 h, about 75% loss of activity Thermoplasma acidophilum
75
-
 1 h, complete loss of activity Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Thermoplasma acidophilum

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 75% of maximal activity, pH 9.5: about 85% of maximal activity Thermoplasma acidophilum

General Information

General Information Comment Organism
physiological function mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
51.5
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum